脱氧核糖核酸中的内部运动II。

Internal motions in deoxyribonucleic acid II.

作者信息

Hogan M E, Jardetzky O

出版信息

Biochemistry. 1980 Jul 22;19(15):3460-8. doi: 10.1021/bi00556a009.

PMID:7190834

Abstract

We have measured the 1H, 31P, and 13C NMR parameters of monodisperse deoxyribonucleic acid (DMA) fragments 260 and 140 base pairs long as a function of temperature and solvent viscosity. On the basis of these measurements, we calculate that within a rodlike DNA helix, the base planes, the deoxyribose sugar, and the sugar-phosphate backbone all experience large fast internal motions. The best fit of the data to a two-state model for internal motion specifies for the internal motions the following amplitudes A and correlation times tau: for the base planes, A = +/-20 degrees and tau = 1 X 10(-9) s; for position 2' of the deoxyribose sugar, A = +/-20 to +/-33 degrees and tau = 1 X 10(-9) s; for the P-H vectors of the sugar-phosphate backbone, A = +/-27 degrees and tau = 2.2 X 10(-9) s.

摘要

我们测量了长度为260和140个碱基对的单分散脱氧核糖核酸（DNA）片段的1H、31P和13C核磁共振参数，这些参数是温度和溶剂粘度的函数。基于这些测量结果，我们计算得出，在棒状DNA螺旋结构中，碱基平面、脱氧核糖以及磷酸二酯骨架都经历了快速的大幅度内部运动。数据与内部运动的双态模型的最佳拟合为内部运动指定了以下振幅A和相关时间τ：对于碱基平面，A = +/-20度，τ = 1×10^(-9)秒；对于脱氧核糖的2'位置，A = +/-20至+/-33度，τ = 1×10^(-9)秒；对于磷酸二酯骨架的P-H向量，A = +/-27度，τ = 2.2×10^(-9)秒。