Banda P W, Quart B D
Res Commun Chem Pathol Pharmacol. 1982 Oct;38(1):57-70.
Previous studies with human subjects have shown that chronic alcohol consumption increases the risk of hepatotoxic effects from an overdose of acetaminophen (APAP). Recent studies in humans and with animal models have shown, on the other hand, that an acute dose of alcohol inhibits the development of hepatotoxic effects from an APAP overdose. Our studies in human subjects show that low doses of alcohol consumption may also provide some protection from the hepatotoxicity of APAP overdose. Specifically, we observe that a small dose of alcohol (the equivalent of 1 ounce of ethanol), consumed over a period of one hour prior to the ingestion of APAP, leads to a reduction in APAP-mercapturic acid excretion. The reduction in the excretion of this metabolite extends for a period of up to 12 hours after APAP ingestion, which is longer than the half-life of the alcohol consumed. The changes in APAP-mercapturic acid excretion are easily detected by our chromatography system via a colorimetric reaction with diphenylpicrylhydrazyl (DPPH).
以往对人体受试者的研究表明，长期饮酒会增加对乙酰氨基酚（APAP）过量服用导致肝毒性作用的风险。另一方面，近期针对人类和动物模型的研究表明，急性剂量的酒精会抑制APAP过量服用所导致的肝毒性作用的发展。我们对人体受试者的研究表明，低剂量饮酒也可能为APAP过量服用导致的肝毒性提供一定程度的保护。具体而言，我们观察到，在摄入APAP前一小时内饮用小剂量酒精（相当于1盎司乙醇），会导致APAP-巯基尿酸排泄量减少。这种代谢物排泄量的减少在摄入APAP后长达12小时内持续存在，这比所饮用酒精的半衰期还要长。通过我们的色谱系统，利用与二苯基苦味酰基肼（DPPH）的比色反应，可以轻松检测到APAP-巯基尿酸排泄量的变化。
