Reisbach G, Gebhart E, Cailleau R
Anticancer Res. 1982 Jul-Aug;2(4):257-60.
The rate of sister chromatid exchanges (SCEs) and the profiles of proliferation (= number of first, second, and third cycle metaphases distinguishable by their staining patterns after BrDU substitution for one, two, or three rounds of DNA replication) have been estimated in three (resp. five) metastatic human breast tumor cell lines. Line MDA MB 231 exhibited SCE rates equalling the level of normal cells, while line MDA MB 435 exhibited a slight increase, and line MDA MB 468 a more distinct increase of the SCE rate over the baseline frequency of normal cells. By the BRDU labelling technique the lines 231 and 435 were shown to be rather fast proliferating in culture, while the line 468, and even more the lines 330 and 361 showed a much slower proliferation pattern. From these results it was concluded that increased SCE rates are rather a facultative than an obligatory feature of malignant cells, and that the Br-DU-labelling technique might be suitable for gaining additional information on proliferation characteristics of tumor cells lines.
在三种（分别为五种）转移性人乳腺肿瘤细胞系中，已对姐妹染色单体交换（SCE）率和增殖谱（即通过在一轮、两轮或三轮DNA复制后用溴脱氧尿苷（BrDU）替代后其染色模式可区分的第一、第二和第三周期中期相的数量）进行了评估。MDA MB 231细胞系的SCE率与正常细胞水平相当，而MDA MB 435细胞系略有增加，MDA MB 468细胞系的SCE率相对于正常细胞的基线频率有更明显的增加。通过溴脱氧尿苷（BRDU）标记技术表明，231和435细胞系在培养中增殖相当快，而468细胞系，甚至330和361细胞系显示出慢得多的增殖模式。从这些结果可以得出结论，SCE率增加更像是恶性细胞的一个兼性而非必然特征，并且溴脱氧尿苷（Br - DU）标记技术可能适用于获取有关肿瘤细胞系增殖特征的更多信息。
