Stobetskiĭ V I, Orlova T M, Kurbatov A V, Khapchaev Iu Kh, Grachev V P
Biull Eksp Biol Med. 1982 May;93(5):85-7.
The frequencies of sister chromatid exchanges (SCE) were studied in primary kidney cell culture, diploid cell line 5018, pseudodiploid spontaneously transformed cell line 4647 and hypotetraploid cell line 455 of the African green monkey. Bromodeoxyuridine was used at a concentration of 10 micrograms/ml. The slides were stained with acridine orange and Giemsa for sister chromatid differentiation. In the first three cases, SCE were scored in 100 metaphases of each culture. The distribution of cells from the number of SCE differs from the normal law and was approximated fairly well by the lognormal distribution pattern. The geometrical means of SCE per cell were 6.90 for cells in primary culture, 9.26 for cells of diploid line 5018; 4.50 for cells of pseudodiploid transformed line 4647 and 9.76 for cells of hypotetraploid transformed line 455.
在非洲绿猴的原代肾细胞培养物、二倍体细胞系5018、假二倍体自发转化细胞系4647和亚四倍体细胞系455中研究了姐妹染色单体交换（SCE）的频率。使用浓度为10微克/毫升的溴脱氧尿苷。玻片用吖啶橙和吉姆萨染色以进行姐妹染色单体分化。在前三种情况下，在每种培养物的100个中期相中对SCE进行计数。细胞中SCE数量的分布不同于正态分布规律，并且通过对数正态分布模式能较好地拟合。原代培养细胞中每个细胞的SCE几何平均值为6.90，二倍体细胞系5018的细胞为9.26；假二倍体转化细胞系4647的细胞为4.50，亚四倍体转化细胞系455的细胞为9.76。
