Schneider E L, Nakanishi Y, Lewis J, Sternberg H
Cancer Res. 1981 Dec;41(12 Pt 1):4973-5.
The bromodeoxyuridine differential chromatid labeling techniques were applied to the simultaneous in vivo examination of cell cycle kinetics and sister chromatid exchange (SCE) induction by various cancer chemotherapeutic agents in normal and Ehrlich ascites tumor cell populations. Compounds which yielded high ratios of SCE (in tumor cells):SCE (in normal cells) also produced greater inhibition of cellular replication in tumor cells when compared to normal cells. Thus, this approach permits in vivo assessment of induction of DNA damage by SCE analysis as well as inhibition of cellular replication by specific cancer chemotherapeutic agents in tumor and normal cell populations.
采用溴脱氧尿苷差异染色单体标记技术，在体内同时检测各种癌症化疗药物对正常细胞群体和艾氏腹水瘤细胞群体的细胞周期动力学及姐妹染色单体交换（SCE）诱导情况。与正常细胞相比，那些在肿瘤细胞中产生高SCE比例（相对于正常细胞中的SCE）的化合物，对肿瘤细胞的细胞复制也有更强的抑制作用。因此，这种方法能够通过SCE分析在体内评估DNA损伤的诱导情况，以及特定癌症化疗药物对肿瘤细胞群体和正常细胞群体细胞复制的抑制作用。
