Cohen A, van Deursen A, van Andel J G, Breure A M
Antonie Van Leeuwenhoek. 1982;48(4):337-52. doi: 10.1007/BF00418287.
A mineral salts medium containing 1% (w/v) glucose was subjected to anaerobic digestion in an upflow reactor. Performance with respect to utilization of glucose was monitored by collection of fermentation gases and calculation of carbon mass balances. Sub-samples of bacterial suspensions from the upflow reactor were incubated with (U-14C)-glucose, (U-14C)-acetate, (2-14C)-propionate, (1-14C)-butyrate or 14C-carbonate. Individual radioactive products in samples from incubation mixtures were analysed by radio gas chromatography. Quantitatively, acetate and propionate were the only important intermediates in glucose degradation by glucose-adapted sludge, with acetate accounting for the largest part of intermediary fatty acid flux.
含有1%（重量/体积）葡萄糖的矿物盐培养基在升流式反应器中进行厌氧消化。通过收集发酵气体和计算碳质量平衡来监测葡萄糖利用方面的性能。将来自升流式反应器的细菌悬浮液子样本与（U-14C）-葡萄糖、（U-14C）-乙酸盐、（2-14C）-丙酸盐、（1-14C）-丁酸盐或14C-碳酸盐一起培养。通过放射性气相色谱法分析培养混合物样本中的各个放射性产物。从数量上看，乙酸盐和丙酸盐是适应葡萄糖的污泥降解葡萄糖过程中仅有的重要中间产物，乙酸盐占中间脂肪酸通量的最大部分。
