Adams D J, Spendlove J C, Spendlove R S, Barnett B B
Appl Environ Microbiol. 1982 Oct;44(4):903-8. doi: 10.1128/aem.44.4.903-908.1982.
The aerosol stability of two particle forms, infectious and potentially infectious, of reovirus were examined under static conditions for a range of relative humidities at 21 and 24 degrees C. Virus aerosolization efficiency was determined for two methods of dissemination: Collison nebulizer and Chicago atomizer. Suspensions of Bacillus subtilis var. niger spores were added to reovirus preparations that included both particle forms and disseminated into a dynamic aerosol toroid to estimate the physical decay of the aerosols. At 90 to 100% relative humidity, both reovirus particle forms showed less than 10-fold loss of infectivity after 12 h of aging. At lower relative humidities the aerosol decay curve showed rapid initial decay followed by a markedly lower decay rate. Our findings reveal that reovirus particles are relatively stable in the airborne state.
在21摄氏度和24摄氏度下，在一系列相对湿度的静态条件下，检测了呼肠孤病毒两种颗粒形式（传染性和潜在传染性）的气溶胶稳定性。通过两种传播方法确定病毒雾化效率：碰撞雾化器和芝加哥雾化器。将枯草芽孢杆菌黑色变种孢子悬浮液添加到包含两种颗粒形式的呼肠孤病毒制剂中，并传播到动态气溶胶环面中，以估计气溶胶的物理衰减。在相对湿度为90%至100%时，两种呼肠孤病毒颗粒形式在老化12小时后感染性损失均小于10倍。在较低的相对湿度下，气溶胶衰减曲线显示出初始快速衰减，随后衰减速率明显降低。我们的研究结果表明，呼肠孤病毒颗粒在空气传播状态下相对稳定。
