SPENDLOVE R S, SCHAFFER F L
J Bacteriol. 1965 Mar;89(3):597-602. doi: 10.1128/jb.89.3.597-602.1965.
Spendlove, R. S. (California State Department of Public Health, Berkeley), and F. L. Schaffer. Enzymatic enhancement of infectivity of reovirus. J. Bacteriol. 89:597-602. 1965.-Enhancement of infectivity by chymotrypsin treatment has been demonstrated with all three types of reovirus, although not in all viral preparations. Enzyme treatment did not produce a simultaneous increase in the hemagglutinating activity of reovirus type 1 (the only type tested). The infectivity of reovirus type 1 (Lang strain) was increased by treatment with chymotrypsin, trypsin, papain, and a filtrate from a culture of a Pseudomonas sp. but not by treatment with pepsin. Sedimentation experiments showed that the property of enhanceability was closely associated with the virus particles themselves. Results of studies involving various sequential treatments with chymotrypsin, and with heat in the presence or absence of 2 m MgCl(2), were compatible with the interpretation that inhibited virus is resistant to exposure to a temperature of 56 C in the absence of MgCl(2), whereas activated virus is thermolabile. Activation of reovirus infectivity by heat in the presence of MgCl(2) and by chymotrypsin was not additive.
斯彭德洛夫，R.S.（加利福尼亚州公共卫生部，伯克利）和F.L.谢弗。胰凝乳蛋白酶增强呼肠孤病毒的感染性。《细菌学杂志》89:597 - 602。1965年。——已证明用胰凝乳蛋白酶处理可增强所有三种类型呼肠孤病毒的感染性，不过并非在所有病毒制剂中都如此。酶处理并未使1型呼肠孤病毒（唯一测试的类型）的血凝活性同时增加。用胰凝乳蛋白酶、胰蛋白酶、木瓜蛋白酶以及一种假单胞菌属培养物的滤液处理可增强1型呼肠孤病毒（朗株）的感染性，但用胃蛋白酶处理则无此效果。沉降实验表明，可增强性这一特性与病毒颗粒本身密切相关。涉及用胰凝乳蛋白酶以及在有或无2 m MgCl₂存在下加热进行各种顺序处理的研究结果符合以下解释：在无MgCl₂时，受抑制的病毒对暴露于56℃温度具有抗性，而活化的病毒对热不稳定。在MgCl₂存在下加热和用胰凝乳蛋白酶对呼肠孤病毒感染性的激活并非相加的。
