Sattar S A, Ijaz M K, Johnson-Lussenburg C M, Springthorpe V S
Appl Environ Microbiol. 1984 Apr;47(4):879-81. doi: 10.1128/aem.47.4.879-881.1984.
Rotavirus SA11, suspended in tryptose phosphate broth with 2.5 mg of rhodamine B per ml, was aerosolized (Collison nebulizer) into a rotating drum, and the aerosols were held at 20 +/- 1 degree C with the desired relative humidity (RH). An all-glass impinger with tryptose phosphate broth was used to collect 1-min (5.6-liter) samples of air from the drum. The virus was found to survive best at medium (50 +/- 5%) RH, where its half-life was nearly 40 h. The half-life of the virus at the low (25 +/- 5%) RH level was about 9 h. Even at 72 h of aerosol age, 45 and 21% of the infectious virus remained detectable in the air at the medium and low RH levels, respectively. The high (80 +/- 5%) RH level was found to be the least favorable to the survival of the virus, since 50% of the infectious virus became undetectable within 2 h of aerosolization. In a separate experiment at the midrange RH, 3% of the infectious virus was detectable in the drum air after 223 h (9 days) of aerosol age. Rotaviruses could, therefore, survive in air for prolonged periods, thus making air a possible vehicle for their dissemination.
将每毫升含2.5毫克罗丹明B的磷酸胰蛋白胨肉汤中悬浮的轮状病毒SA11，通过雾化器（科里森雾化器）雾化到一个转鼓中，气溶胶在20±1摄氏度及所需相对湿度（RH）下保存。使用装有磷酸胰蛋白胨肉汤的全玻璃冲击式采样器从转鼓中采集1分钟（5.6升）的空气样本。发现该病毒在中等相对湿度（50±5%）下存活最佳，其半衰期接近40小时。在低相对湿度（25±5%）水平下，病毒的半衰期约为9小时。即使在气溶胶形成72小时后，在中等和低相对湿度水平的空气中仍分别有45%和21%的感染性病毒可被检测到。发现高相对湿度（80±5%）水平对病毒存活最不利，因为在雾化2小时内50%的感染性病毒就无法检测到了。在另一个中等相对湿度的单独实验中，在气溶胶形成223小时（9天）后，转鼓空气中3%的感染性病毒仍可被检测到。因此，轮状病毒可在空气中长时间存活，从而使空气成为其传播的可能载体。
