Ivankovic S, Frank N, Filipovic N, Haupt P, Wiessler M
Arch Geschwulstforsch. 1982;52(5):355-61.
Following monotherapy with cyclophosphamide rat leukemia cells (L 5222) survive only in the CNS, as has been described previously. Here we report on investigations performed to elucidate the influence of rat brain tissue on cyclophosphamide activity. It was found that the toxicity of cyclophosphamide is enhanced 5 fold after incubation with freshly prepared brain homogenate. In parallel experiments it was also found that cyclophosphamide preincubated with brain homogenates is therapeutically less effective toward L 5222 cells than cyclophosphamide alone. The incubation of cyclophosphamide with liver homogenate did not result in the same enhancement of cyclophosphamide toxicity nor in the decrease of therapeutic effects. The different influence of the two tissues on cyclophosphamide suggests two different metabolic mechanisms.
正如之前所描述的，用环磷酰胺进行单一疗法后，大鼠白血病细胞（L 5222）仅在中枢神经系统中存活。在此，我们报告为阐明大鼠脑组织对环磷酰胺活性的影响而进行的研究。结果发现，环磷酰胺与新鲜制备的脑匀浆孵育后，其毒性增强了5倍。在平行实验中还发现，与脑匀浆预孵育的环磷酰胺对L 5222细胞的治疗效果不如单独使用环磷酰胺。环磷酰胺与肝匀浆孵育既未导致环磷酰胺毒性的相同增强，也未导致治疗效果的降低。两种组织对环磷酰胺的不同影响表明存在两种不同的代谢机制。
