兔网织红细胞从双铁兔血清转铁蛋白中去除铁。

Removal of iron from diferric rabbit serum transferrin by rabbit reticulocytes.

作者信息

Heaphy S, Williams J

出版信息

Biochem J. 1982 Sep 1;205(3):619-21. doi: 10.1042/bj2050619.

DOI:10.1042/bj2050619

PMID:7150235

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1158529/

Abstract

When radioiron-labelled transferrin with 55Fe located predominantly in the N-terminal iron-binding site and 59Fe predominantly in the C-terminal iron-binding site was incubated with rabbit reticulocytes, both radioisotopes of iron were removed at similar rates. Electrophoresis of transferrin samples taken during the course of an incubation, in polyacrylamide gels containing 6 M-urea, showed that iron was removed in a pairwise fashion, giving rise to iron-free transferrin.

摘要

当主要位于N端铁结合位点的含55Fe的放射性铁标记转铁蛋白和主要位于C端铁结合位点的含59Fe的放射性铁标记转铁蛋白与兔网织红细胞一起孵育时，两种铁的放射性同位素以相似的速率被去除。在含有6M尿素的聚丙烯酰胺凝胶中对孵育过程中取出的转铁蛋白样品进行电泳，结果表明铁是以两两配对的方式被去除的，从而产生了无铁转铁蛋白。