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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Van der Heul C, Kroos M J, Van Noort W L, Van Eijk H G

Clin Sci (Lond). 1981 Feb;60(2):185-90. doi: 10.1042/cs0600185.

According to the Fletcher-Huehns hypothesis there exists a functional difference between the two iron-binding sites of transferrin. 2. The aim of the study presented was to evaluate this hypothesis in a homogeneous system, with human bone marrow cells and pure human monoferric transferrins A and B. 3. For this reason normal human bone marrow cells were incubated with human monoferric transferrin. The monoferric transferrins A and B were obtained by selective labelling at different pH of apotransferrin followed by preparative isoelectric focusing in granulated gels. The uptake of iron by the cell suspensions from monoferric transferrins A and B was equal. 4. In a heterogeneous but more active system for the removal of iron from human transferrin in vitro the two human monoferric transferrins did not show any significant functional differences. 5. No support for the Fletcher-Huehns hypothesis could be obtained.

根据弗莱彻 - 许恩斯假说，转铁蛋白的两个铁结合位点存在功能差异。2. 本研究的目的是在一个同质系统中，用人骨髓细胞以及纯人单铁转铁蛋白A和B来评估这一假说。3. 因此，将正常人骨髓细胞与人单铁转铁蛋白一起孵育。单铁转铁蛋白A和B是通过在不同pH值下对脱铁转铁蛋白进行选择性标记，然后在颗粒凝胶中进行制备性等电聚焦获得的。细胞悬液从单铁转铁蛋白A和B中摄取铁的量是相等的。4. 在一个异质但更具活性的体外从人转铁蛋白中去除铁的系统中，这两种人单铁转铁蛋白未显示出任何显著的功能差异。5. 无法获得对弗莱彻 - 许恩斯假说的支持。

Van der Heul C, Kroos M J, Van Noort W L, Van Eijk H G

Clin Sci (Lond). 1981 Feb;60(2):185-90. doi: 10.1042/cs0600185.

According to the Fletcher-Huehns hypothesis there exists a functional difference between the two iron-binding sites of transferrin. 2. The aim of the study presented was to evaluate this hypothesis in a homogeneous system, with human bone marrow cells and pure human monoferric transferrins A and B. 3. For this reason normal human bone marrow cells were incubated with human monoferric transferrin. The monoferric transferrins A and B were obtained by selective labelling at different pH of apotransferrin followed by preparative isoelectric focusing in granulated gels. The uptake of iron by the cell suspensions from monoferric transferrins A and B was equal. 4. In a heterogeneous but more active system for the removal of iron from human transferrin in vitro the two human monoferric transferrins did not show any significant functional differences. 5. No support for the Fletcher-Huehns hypothesis could be obtained.

根据弗莱彻 - 许恩斯假说，转铁蛋白的两个铁结合位点存在功能差异。2. 本研究的目的是在一个同质系统中，用人骨髓细胞以及纯人单铁转铁蛋白A和B来评估这一假说。3. 因此，将正常人骨髓细胞与人单铁转铁蛋白一起孵育。单铁转铁蛋白A和B是通过在不同pH值下对脱铁转铁蛋白进行选择性标记，然后在颗粒凝胶中进行制备性等电聚焦获得的。细胞悬液从单铁转铁蛋白A和B中摄取铁的量是相等的。4. 在一个异质但更具活性的体外从人转铁蛋白中去除铁的系统中，这两种人单铁转铁蛋白未显示出任何显著的功能差异。5. 无法获得对弗莱彻 - 许恩斯假说的支持。