Creeth J M, Harding S E
Biochem J. 1982 Sep 1;205(3):639-41. doi: 10.1042/bj2050639.
A simple check for the presence of heterogeneity in a macromolecular system is proposed, employing comparison of Rayleigh sedimentation-equilibrium patterns for two solutions of the same fringe concentration but differing absolute concentrations. The method is illustrated by application to a bronchial glycoprotein from a cystic-fibrosis patient.
提出了一种用于检测大分子系统中是否存在异质性的简单方法,即比较两种具有相同条纹浓度但绝对浓度不同的溶液的瑞利沉降平衡模式。通过将该方法应用于一名囊性纤维化患者的支气管糖蛋白来说明。