Banerjee A, Turner A J, Guha S R
Biochem Pharmacol. 1982 Oct 15;31(20):3219-23. doi: 10.1016/0006-2952(82)90553-6.
The crude mitochondrial fraction of rat brain contains an active dehydrogenase involved in the direct oxidation of gamma-aminobutyric acid. INT (p-iodonitrotetrazolium violet) can serve as an efficient acceptor of electrons in this dehydrogenase reaction. During this oxidation of GABA, ammonia is not produced. In vitro the dehydrogenase activity is inhibited by certain MAO inhibitors. The effects of various inhibitors of GABA-T and GAD were also investigated. The dehydrogenase activity was found to be susceptible to various anti-convulsants and inhibitors of electron transport. The co-factors which may be involved in the transfer of electrons during GABA oxidation in the presence of INT are also discussed.
大鼠脑的粗线粒体部分含有一种参与γ-氨基丁酸直接氧化的活性脱氢酶。INT（对碘硝基四氮唑紫）可作为该脱氢酶反应中有效的电子受体。在γ-氨基丁酸的这种氧化过程中，不会产生氨。在体外，脱氢酶活性受到某些单胺氧化酶抑制剂的抑制。还研究了γ-氨基丁酸转氨酶和谷氨酸脱羧酶的各种抑制剂的作用。发现脱氢酶活性对各种抗惊厥药和电子传递抑制剂敏感。还讨论了在INT存在下γ-氨基丁酸氧化过程中可能参与电子转移的辅助因子。
