Grunze M, Haest C W, Deuticke B
Biochim Biophys Acta. 1982 Dec 8;693(1):237-45. doi: 10.1016/0005-2736(82)90491-6.
Human erythrocytes, incubated with sonicated dispersions of phosphatidylcholine, cholesterol and saturated straight-chain alcohols (C16-C18) develop stiff, rod-shaped, hemoglobin-containing membrane projections within 120 min. The number of these 'rods' varies (1-3 per cell), they reach a length of up to 14 microns (twice the cell diameter) and a thickness of 0.3-1.0 microns. 'Rods' may be separated from 'residual cells' by shear flow and centrifugation without severe hemolysis. Lipid analyses carried out on residual cells and rods indicate lateral segregation of the phospholipids of the outer leaf of the membrane lipid bilayer (phosphatidylcholine and sphingomyelin) and of the alcohol applied. Phosphatidylcholine accumulates in the residual cells, sphingomyelin and the alcohol in the rods. No differences in membrane protein patterns were observed between rods and residual cells. The rod-shape is dependent on the presence of the alcohol, extraction of the alcohol converts rods into hemoglobin-containing spheres without lysis. The formation of rods, which is indicative of a lateral phase separation, is discussed in terms of lipid-lipid interactions and with respect to parameters determining the shape of cells.
将人红细胞与磷脂酰胆碱、胆固醇和饱和直链醇（C16 - C18）的超声分散液一起孵育，在120分钟内红细胞会形成坚硬的、含血红蛋白的杆状膜突起。这些“杆状物”的数量各不相同（每个细胞1 - 3个），它们的长度可达14微米（细胞直径的两倍），厚度为0.3 - 1.0微米。“杆状物”可通过剪切流和离心从“残余细胞”中分离出来，且不会造成严重溶血。对残余细胞和杆状物进行的脂质分析表明，膜脂双层外叶的磷脂（磷脂酰胆碱和鞘磷脂）以及所施加的醇发生了横向分离。磷脂酰胆碱积聚在残余细胞中，鞘磷脂和醇积聚在杆状物中。在杆状物和残余细胞之间未观察到膜蛋白模式的差异。杆状形状取决于醇的存在，去除醇会使杆状物转化为含血红蛋白的球体而不发生裂解。从脂质 - 脂质相互作用以及决定细胞形状的参数方面讨论了指示横向相分离的杆状物的形成。
