Ferrell J E, Lee K J, Huestis W H
Biochemistry. 1985 Jun 4;24(12):2849-57. doi: 10.1021/bi00333a006.
When human erythrocytes are incubated with certain phospholipids, the cells become spiculate echinocytes, resembling red cells subjected to metabolic starvation or Ca2+ loading. The present study examines (1) the mode of binding of saturated phosphatidylcholines and egg lysophosphatidylcholine to erythrocytes and (2) the quantitative relationship between phospholipid incorporation and red cell shape. We find that the phospholipids studied become intercalated into erythrocyte membranes, not simply adsorbed to the cell surface. Spin-labeling and radiolabeling data show that the incorporation of (4 +/- 1) X 10(6) molecules of exogenous phosphatidylcholine per cell converts discocytes to stage 3 echinocytes with about 35 conical spicules. This amount of lipid incorporation is estimated to expand the red cell membrane outer monolayer by 1.7% +/- 0.6%. Calculations of the inner and outer monolayer surface areas of model discocytes and stage 3 echinocytes yield an estimated difference of 0.7% +/- 0.2%.
当人类红细胞与某些磷脂一起孵育时，细胞会变成有刺的棘状红细胞，类似于经历代谢饥饿或钙离子加载的红细胞。本研究考察了：（1）饱和磷脂酰胆碱和鸡蛋溶血磷脂酰胆碱与红细胞的结合方式；（2）磷脂掺入与红细胞形状之间的定量关系。我们发现，所研究的磷脂会插入红细胞膜中，而不仅仅是吸附在细胞表面。自旋标记和放射性标记数据表明，每个细胞掺入（4±1）×10⁶个外源磷脂酰胆碱分子会将双凹圆盘状红细胞转变为具有约35个锥形刺的3期棘状红细胞。据估计，这种脂质掺入量会使红细胞膜外层单分子层扩大1.7%±0.6%。对模型双凹圆盘状红细胞和3期棘状红细胞的内层和外层单分子层表面积进行计算，得出的估计差异为0.7%±0.2%。
