Mortensen P B, Kølvraa S, Gregersen N, Rasmussen K
Biochim Biophys Acta. 1982 Nov 12;713(2):393-7. doi: 10.1016/0005-2760(82)90258-2.
The beta-oxidation rate of dodecanedioic acid in rat liver homogenates (600 X g supernatant fraction) was determined by simultaneous measurements of the C6-C12-dicarboxylic acids, i.e., adipic, suberic, sebacic and dodecanedioic acids, in relation to time in assays incubated with dodecanedioic acid. Measurements were performed by a combined gas chromatographic-mass spectrometric technique, i.e., selected ion-monitoring. The beta-oxidation rate was registered as the consumption rate of dodecanedioic acid and as the initial rise in the concentrations of C6-C10-dicarboxylic acids. The beta-oxidation rate of C8-C12-dicarboxylic acids was increased many times in homogenates from clofibrate-treated rats. Moreover, it was unexpectedly found that 2.0 mM cyanide was unable to inhibit the beta-oxidation rate of the dicarboxylic acids in vitro, but in fact caused a minor increase in the rate of beta-oxidation in homogenates from both normal and clofibrate-treated rats. It was concluded that the present results strongly indicate the existence of a peroxisomal beta-oxidation of dicarboxylic acids.
通过同时测量与十二烷二酸一起孵育的测定中C6 - C12 - 二羧酸（即己二酸、辛二酸、癸二酸和十二烷二酸）随时间的变化，来确定大鼠肝脏匀浆（600×g上清液部分）中十二烷二酸的β - 氧化速率。测量采用气相色谱 - 质谱联用技术，即选择离子监测法进行。β - 氧化速率记录为十二烷二酸的消耗速率以及C6 - C10 - 二羧酸浓度的初始上升。在氯贝丁酯处理的大鼠的匀浆中，C8 - C12 - 二羧酸的β - 氧化速率增加了许多倍。此外，意外发现2.0 mM氰化物在体外不能抑制二羧酸的β - 氧化速率，但实际上在正常和氯贝丁酯处理的大鼠的匀浆中导致β - 氧化速率略有增加。得出的结论是，目前的结果有力地表明存在二羧酸的过氧化物酶体β - 氧化。
