Saito T, Itoh T, Adachi S, Suzuki T, Usui T
Biochim Biophys Acta. 1982 Nov 24;719(2):309-17. doi: 10.1016/0304-4165(82)90104-0.
The carbohydrate units linked to caseinoglycopeptide from colostrum taken 30 min after parturition were released as reduced oligosaccharides by alkaline borohydride treatment, and separated into four acidic oligosacchariditols (a hexa- (11.0%), penta- (38.5%), tetra- (35.5%) and a trisacchariditol (15.0%)). Structural studies showed that the hexasacchariditol was a new structure and had the chemical structure: NeuNAc-alpha-2-3-Gal-beta-1-3-[NeuNAc-alpha-2-3-Gal- beta-1-4-GlcNAc-beta-1-6-]-N-acetylgalactosaminitol(GalNAcItol). The other three oligosacchariditols were shown to be NeuNAc-alpha-2-3-Gal-beta-1-3-[Gal-beta-1-4-GlcNAc-beta-1-6-]-GalNAcItol, NeuNAc-alpha-2-3-Gal-beta-1-3-[NeuNAc-alpha-2-6-]-GalNAcItol and NeuNAc-alpha-2-3-Gal-beta-1-3-GalNAcItol, which were identical with those found previously in colostrum kappa-casein taken 6 h after parturition.
