Saito T, Itoh T, Adachi S
Laboratory of Food Hygiene, Faculty of Social Welfare, Tohoku Fukushi University, Sendai, Japan.
Carbohydr Res. 1987 Jul 15;165(1):43-51. doi: 10.1016/0008-6215(87)80076-9.
Three neutral trisaccharides, which comprise 25.1% of the neutral oligosaccharide other than lactose, were isolated from bovine colostrum, obtained 6 h after parturition, by l.c. on amino silica gel. The chemical structures were identified, by methylation analysis with direct m.s. and g.l.c.-m.s., and by structural analysis with 13C-n.m.r., as beta-D-Galp-(1----4)-[alpha-L-Fucp-(1----3)-]-D-GlcNAc (3-fucosyl-N-acetyllactosamine), beta-D-Galp-(1----3)-beta-D-Galp-(1----4)-D-Glc (3'-galactosyllactose), and beta-D-Galp-(1----6)-beta-D-Galp-(1----4)-D-Glc (6'-galactosyllactose). The The first-named compound was a novel oligosaccharide from mammalian milk.
从分娩后6小时获取的牛初乳中，通过在氨基硅胶上进行液相色谱法分离出三种中性三糖，它们占除乳糖外中性寡糖的25.1%。通过直接质谱和气相色谱 - 质谱联用的甲基化分析以及通过13C - 核磁共振进行结构分析，确定其化学结构为β - D - 半乳糖基 - (1→4)-[α - L - 岩藻糖基 - (1→3)-]-D - 葡萄糖胺（3 - 岩藻糖基 - N - 乙酰乳糖胺）、β - D - 半乳糖基 - (1→3)-β - D - 半乳糖基 - (1→4)-D - 葡萄糖（3'- 半乳糖基乳糖）和β - D - 半乳糖基 - (1→6)-β - D - 半乳糖基 - (1→4)-D - 葡萄糖（6'- 半乳糖基乳糖）。上述第一种化合物是一种来自哺乳动物乳汁的新型寡糖。
