Miroshnichenko M L, Smirnova O V, Smirnov A N, Rozen V B
Biull Eksp Biol Med. 1982 Nov;94(11):90-2.
The ability of 57 steroids and their analogs to compete with 3H-estradiol for binding sites of an unusual estrogen-binding protein (UEBP) of the male rat liver was studied. The magnitudes of the equilibrium constants of association of 3H-estradiol, 3H-estriol, and 3H-5 alpha-androstane-3 alpha, 17 beta-diol with the UEBP were measured. It was shown that the derivatives of estrane and androstane, in contrast to those of pregnane, stilbene and triphenylethane, can effectively interact with the UEBP. The ability of estrogens and androgens to interact with the UEBP primarily depends on oxygen functions of the A and D rings and on general conformation of the steroid molecule. It is suggested that there are 2 subcenters for the steroid A and D rings both at the estrogen and androgen sites of the UEBP molecule. These subcenters for the two types of sex steroids either do not coincide or coincident are only subcenters for the ligand A rings.
研究了57种甾体及其类似物与3H-雌二醇竞争雄性大鼠肝脏中一种特殊雌激素结合蛋白（UEBP）结合位点的能力。测定了3H-雌二醇、3H-雌三醇和3H-5α-雄甾烷-3α,17β-二醇与UEBP结合的平衡常数大小。结果表明，与孕甾烷、二苯乙烯和三苯乙烷的衍生物相比，雌甾烷和雄甾烷的衍生物能有效地与UEBP相互作用。雌激素和雄激素与UEBP相互作用的能力主要取决于A环和D环的氧官能团以及甾体分子的总体构象。提示在UEBP分子的雌激素和雄激素位点上，甾体A环和D环均有2个亚中心。这两类性甾体的这些亚中心要么不重合，要么重合的只是配体A环的亚中心。
