Ryall R G, Rumpff J
Ann Clin Biochem. 1986 Mar;23 ( Pt 2):172-9. doi: 10.1177/000456328602300206.
The effect of changes in sample isoferritin composition on the behaviour of ferritin 2-site immunoradiometric assays was investigated using a computer model and experimental studies. Modelling studies predicted that under conditions where a sample has a different isoferritin composition from the assay standards, progressively higher values will be generated for the apparent ferritin content of the sample when it is analysed at increasing dilutions. In addition, the assay results will underestimate the actual ferritin content of such samples at all dilutions. Dilutional discrepancies in assay results and underestimation of sample ferritin concentrations were found in practical assays when synthetic samples with demonstrably different isoferritin profiles were analysed. Differences in isoferritin composition between assay standards and assayed samples may therefore be a cause of dilutional discrepancies in some results from ferritin 2-site immunoradiometric assays.
利用计算机模型和实验研究，探讨了样品异铁蛋白组成变化对铁蛋白双位点免疫放射分析行为的影响。模型研究预测，在样品异铁蛋白组成与分析标准不同的条件下，当对样品进行递增稀释分析时，其表观铁蛋白含量将逐渐产生更高的值。此外，在所有稀释度下，分析结果都会低估此类样品的实际铁蛋白含量。当分析具有明显不同异铁蛋白谱的合成样品时，在实际分析中发现了分析结果的稀释差异以及样品铁蛋白浓度的低估。因此，分析标准与被分析样品之间异铁蛋白组成的差异可能是铁蛋白双位点免疫放射分析某些结果出现稀释差异的原因。
