正常对照者和患有各种疾病的患者血清I型和II型胃蛋白酶原的放射免疫测定

Radioimmunoassay of serum group I and group II pepsinogens in normal controls and patients with various disorders.

作者信息

Ichinose M, Miki K, Furihata C, Kageyama T, Hayashi R, Niwa H, Oka H, Matsushima T, Takahashi K

出版信息

Clin Chim Acta. 1982 Dec 9;126(2):183-91. doi: 10.1016/0009-8981(82)90034-1.

Abstract

A radioimmunoassay (RIA) for human group I pepsinogens (PgI) in serum was developed, using PgI purified from gastric mucosa. The sensitivity (0.7 micrograms/l) and reproducibility of the assay were satisfactory for clinical use. In normal controls, total serum pepsinogen (T-Pg) level was 58.9 +/- 31.7 micrograms/l (mean +/- SD) (PgI, 43.6 +/- 25.0 micrograms/l; PgII, 15.3 +/- 11.1 micrograms/l). Peptic ulcer cases had elevated T-Pg levels (gastric ulcer, gastroduodenal ulcer and duodenal ulcer, in increasing order of magnitude). T-Pg levels were not useful for diagnosis of peptic ulcer because of a large overlap with normal controls. T-Pg levels were low in patients with gastric polyp and in aged subjects. In these groups, the decrease of PgI was more marked than that of PgII.

摘要

利用从胃黏膜中纯化得到的I组胃蛋白酶原（PgI），开发了一种用于检测血清中人类I组胃蛋白酶原的放射免疫分析法（RIA）。该检测方法的灵敏度（0.7微克/升）和重现性对于临床应用来说是令人满意的。在正常对照组中，血清总胃蛋白酶原（T-Pg）水平为58.9±31.7微克/升（平均值±标准差）（PgI为43.6±25.0微克/升；PgII为15.3±11.1微克/升）。消化性溃疡患者的T-Pg水平升高（胃溃疡、胃十二指肠溃疡和十二指肠溃疡，程度依次增加）。由于与正常对照组有很大重叠，T-Pg水平对消化性溃疡的诊断没有帮助。胃息肉患者和老年受试者的T-Pg水平较低。在这些组中，PgI的下降比PgII更明显。