细胞色素P450与美替拉酮的复合物。一种定量分析大鼠肝微粒体中苯巴比妥诱导的细胞色素P450的简便方法。
Complexes of cytochrome P450 with metyrapone. A convenient method for the quantitative analysis of phenobarbital-inducible cytochrome P450 in rat liver microsomes.
作者信息
Mitani F, Shephard E A, Phillips I R, Rabin B R
出版信息
FEBS Lett. 1982 Nov 8;148(2):302-6. doi: 10.1016/0014-5793(82)80829-6.
PMID:7152025
Abstract
Phenobarbital-inducible cytochrome P450 variants have a higher affinity (app. Ks = 0.6 microM). The difference in affinity has enabled us to quantitate, by metyrapone-induced difference spectral analysis, phenobarbital-inducible cytochromes P450 in liver microsomal membranes. The result obtained by this simple, rapid technique compare well with those found by more sophisticated techniques such as radioimmunoassay and immunoprecipitation.
摘要
苯巴比妥诱导的细胞色素P450变体具有更高的亲和力（表观解离常数Ks = 0.6微摩尔）。亲和力的差异使我们能够通过甲吡酮诱导的差光谱分析对肝微粒体膜中苯巴比妥诱导的细胞色素P450进行定量。通过这种简单、快速的技术获得的结果与通过放射免疫测定和免疫沉淀等更复杂技术获得的结果相当。
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