Department of Life Science and Biomedical Research Institute, University of Wisconsin-Parkside, Kenosha, Wisconsin 53141.
Plant Physiol. 1984 Jun;75(2):442-6. doi: 10.1104/pp.75.2.442.
N(6)-(Delta(2)-isopentenyl)Adenine and N(6)-(Delta(2)-isopentenyl)adenosine were hydroxylated, respectively, to 6-(4-hydroxy-3-methyl-trans-2-butenylamino)purine and 6-(4-hydroxy-3-methyl-trans-2-butenylamino) -9-beta-ribofuranosylpurine in the presence of NADPH and the microsomal fraction from cauliflowers (Brassica oleracea L.). The hydroxylating reaction was completely inhibited by 10 millimolars metyrapone and partially inactivated by 10-minute treatment of the microsomal preparation with ethylene. The cytokinins were also dealkylated by the microsomal enzymes and formed adenine from cytokinin base and adenosine from cytokinin nucleoside. These results suggest that plant cytochrome P-450 is involved in the conversion of one type of cytokinin to another, and in the modification of cytokinin molecules.
N(6)-(Delta(2)-异戊烯基)腺嘌呤和 N(6)-(Delta(2)-异戊烯基)腺苷分别在 NADPH 和花椰菜( Brassica oleracea L.)的微粒体部分存在的情况下被羟化,形成 6-(4-羟基-3-甲基-trans-2-丁烯基氨基)嘌呤和 6-(4-羟基-3-甲基-trans-2-丁烯基氨基)-9-beta-核糖基嘌呤。羟化反应被 10 毫摩尔甲吡酮完全抑制,10 分钟的乙烯处理部分失活。细胞分裂素也被微粒体酶脱烷基化,并从细胞分裂素碱基形成腺嘌呤,从细胞分裂素核苷形成腺苷。这些结果表明,植物细胞色素 P-450 参与了一种细胞分裂素向另一种细胞分裂素的转化,以及细胞分裂素分子的修饰。