Johnson T A, Mercer R R, Taylor P C, Graham J A, O'Neil J J
J Appl Physiol Respir Environ Exerc Physiol. 1982 Dec;53(6):1634-7. doi: 10.1152/jappl.1982.53.6.1634.
We have developed and tested an automated system that measures in vitro oxygen consumption by Warburg manometry in as many as 16 units that are under the simultaneous control of a microcomputer which requires attention at the beginning of the study only. The all-glass Submarine Volumometers used are readily adapted to automation using a microcomputer that interacts with an infrared photodetector sensitive to manometric changes in the reaction vessel and a stepper motor that can advance the calibrated micrometer in response to these changes. The microcomputer interacts with the user at the start of the study during data entry and subsequently determines volume changes related to oxygen consumption, calculates respiration rates, and prints or graphs the results without further user interaction. We compared this automated system with manual methods by measuring the oxygen consumption of lung tissue slices and by determining the ability of the system to match known volumes entered manually. We found that the results obtained using the automated system were not significantly different from known manual methods (P less than 0.05).
我们已经开发并测试了一种自动化系统，该系统通过瓦氏测压法测量多达16个单元的体外氧消耗，这些单元由一台微型计算机同时控制，且仅在研究开始时需要人工关注。所使用的全玻璃潜水艇式体积计很容易通过微型计算机实现自动化，该微型计算机与一个对反应容器中压力变化敏感的红外光电探测器以及一个能根据这些变化推进校准千分尺的步进电机进行交互。在研究开始时数据输入期间，微型计算机与用户进行交互，随后确定与氧消耗相关的体积变化，计算呼吸速率，并在无需用户进一步交互的情况下打印结果或绘制图表。我们通过测量肺组织切片的氧消耗以及确定该系统匹配手动输入的已知体积的能力，将这个自动化系统与手动方法进行了比较。我们发现，使用自动化系统获得的结果与已知的手动方法没有显著差异（P小于0.05）。
