Horstmann M, Opitz B, Opitz K
J Pharmacol Methods. 1985 Sep;14(2):119-28. doi: 10.1016/0160-5402(85)90049-x.
A closed-circuit system for the simultaneous measurement of oxygen consumption in six small laboratory animals is described. The system consisted of six glass chambers in a big water bath of constant temperature, an oxygen-supply unit, six pressure sensors, a microcomputer and a printer, or, optionally, a desk-top calculator. Ventilation within the chambers provided rapid equilibration of air temperature and humidity conditions. Easily sealing glass lids facilitated operation and maintenance of the system. Carbon dioxide was absorbed inside the chambers by soda lime. At 6-min intervals the consumed oxygen in each chamber was automatically replaced with oxygen from a low-pressure tank. The microcomputer, a self-constructed piece of equipment containing a Z 80 A central processing unit, received pressure data from the separately operating sensors, determined the decrements in chamber pressure due to oxygen consumption by the animal sitting in the chamber, and stored the calculated values in a 2 Kbyte RAM for outprint at the user's request. The apparatus proved to be sensitive, accurate, and easy to operate.
本文描述了一种用于同时测量六只小型实验动物耗氧量的闭路系统。该系统由置于恒温大水槽中的六个玻璃室、一个氧气供应单元、六个压力传感器、一台微型计算机和一台打印机组成，或者也可以选用一台台式计算器。室内通风可使空气温度和湿度条件快速达到平衡。易于密封的玻璃盖便于系统的操作和维护。室内的二氧化碳由苏打石灰吸收。每隔6分钟，每个室内消耗的氧气会自动由一个低压罐补充。微型计算机是一台自行组装的设备，包含一个Z 80 A中央处理器，它从单独运行的传感器接收压力数据，确定由于室内动物耗氧导致的室内压力下降，并将计算值存储在一个2千字节的随机存取存储器中，以便根据用户要求输出打印。该装置被证明是灵敏、准确且易于操作的。
