Curvall M, Kazemi-Vala E, Enzell C R
J Chromatogr. 1982 Nov 12;232(2):283-93. doi: 10.1016/s0378-4347(00)84168-7.
A rapid and sensitive method is described for the stimultaneous determination of nicotine and its principal metabolite, cotinine, in plasma. A one-step extraction procedure is employed and the quantitative analyses are performed by capillary column gas chromatography using a thermionic specific detector. Other special measures to avoid contamination from external sources such as atmosphere, solvents and laboratory equipment, which constitutes the major limiting factor of nicotine assay, were also undertaken. The structural analogues of nicotine and cotinine, N-methylanabasine and N-ethylnorcotinine, are used as internal standards. Moreover, a micromethod, which requires only 0.1 ml of plasma and found to be suitable for analysis of cotinine in finger-tip samples of blood, is described. LInearity over the concentration ranges 5-100 ng of nicotine per ml of plasma and 5-500 ng of cotinine per ml of plasma is demonstrated. The precision of the method has been investigated by determining the reproducibility at different levels of nicotine and cotinine within the working ranges, for both 1-ml and 0.1-ml samples of plasma.
本文描述了一种快速、灵敏的方法，用于同时测定血浆中的尼古丁及其主要代谢物可替宁。采用一步萃取程序，通过使用热离子特异性检测器的毛细管柱气相色谱法进行定量分析。还采取了其他特殊措施，以避免来自大气、溶剂和实验室设备等外部来源的污染，这些是尼古丁测定的主要限制因素。尼古丁和可替宁的结构类似物N-甲基新烟草碱和N-乙基去甲可替宁用作内标。此外，还描述了一种微量法，该方法仅需0.1 ml血浆，适用于分析指尖血样中的可替宁。在每毫升血浆中尼古丁浓度为5-100 ng和可替宁浓度为5-500 ng的范围内显示出线性关系。通过测定1 ml和0.1 ml血浆样品在工作范围内不同水平的尼古丁和可替宁的重现性，研究了该方法的精密度。
