用75硒代蛋氨酸对病毒蛋白进行体内标记。

In vivo labelling of viral proteins with 75selenomethionine.

作者信息

Enzmann P J

出版信息

J Virol Methods. 1982 Nov;5(3-4):243-6. doi: 10.1016/0166-0934(82)90014-3.

Abstract

A method is described for in vivo labelling of viral proteins with a gamma ray emitting radionuclide. The amino acid methionine labelled with 75Se instead of 35S will be accepted by cells in culture for protein synthesis. A high specific labelling of viral proteins is achieved which allows rapid detection of virus or viral proteins in gradient centrifugation and PAGE as well as further biochemical or serological analysis of the proteins. As an example hog cholera virus (family Togaviridae) was labelled with [75Se]methionine and analyzed in sucrose and CsCl gradients and in PAGE. The results were similar to those obtained by labelling of proteins with [35S]methionine.

摘要

描述了一种用发射γ射线的放射性核素对病毒蛋白进行体内标记的方法。用75Se而非35S标记的甲硫氨酸会被培养细胞接受用于蛋白质合成。可实现病毒蛋白的高特异性标记，这使得在梯度离心和聚丙烯酰胺凝胶电泳（PAGE）中能快速检测病毒或病毒蛋白，以及对这些蛋白进行进一步的生化或血清学分析。例如，用[75Se]甲硫氨酸标记猪霍乱病毒（披膜病毒科），并在蔗糖和氯化铯梯度以及PAGE中进行分析。结果与用[35S]甲硫氨酸标记蛋白所获得的结果相似。