Ordonez G A, Newman J T, Stone M J
J Clin Microbiol. 1982 Oct;16(4):751-3. doi: 10.1128/jcm.16.4.751-753.1982.
A simple and rapid method has been developed for the separation of serum immunoglobulin M (IgM) and IgG. CM Bio-Gel A chromatography was used in the technique, which resulted in an IgM-rich fraction containing 31% of the original serum IgM and less than 2% of the serum IgG. The procedure was used to detect masked IgM antibodies in patients suspected of having Toxoplasma gondii infections.
已开发出一种简单快速的方法来分离血清免疫球蛋白M(IgM)和免疫球蛋白G(IgG)。该技术采用CM Bio-Gel A色谱法,得到了一个富含IgM的组分,其中含有原始血清IgM的31%,而血清IgG含量不到2%。该方法用于检测疑似弓形虫感染患者体内的隐匿性IgM抗体。