从免疫球蛋白G中简易色谱分离免疫球蛋白M及其在弓形虫间接免疫荧光中的应用

Simplified chromatographic separation of immunoglobulin M from G and its application to toxoplasma indirect immunofluorescence.

作者信息

Pyndiah N, Krech U, Price P, Wilhelm J

出版信息

J Clin Microbiol. 1979 Feb;9(2):170-4. doi: 10.1128/jcm.9.2.170-174.1979.

DOI:10.1128/jcm.9.2.170-174.1979

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC272984/

Abstract

The indirect immunofluorescent (IIF) antibody technique for the detection of Toxoplasma gondii immunoglobulin M (IgM) often gives false negative results, probably due to the competition between IgG and IgM. We therefore adapted a gel filtration procedure for the separation of IgG and IgM to a routine diagnostic test capable handling at least 10 sera per day and requiring only 50 microliters of serum. The results from 108 sera having positive complement fixation titers for Toxoplasma showed that 17 were IgM positive when the whole serum was tested by IIF compared with 55 positive when the IgM fraction was used. Sera with antideoxyribonucleic acid titers do not give false positive results after fractionation, and the removal of IgG eliminates false positive results due to rheumatoid factor. A prospective study showed that Toxoplasma IgM may persist up to 9 months.

摘要

用于检测弓形虫免疫球蛋白M（IgM）的间接免疫荧光（IIF）抗体技术常常得出假阴性结果，这可能是由于IgG和IgM之间的竞争所致。因此，我们将一种用于分离IgG和IgM的凝胶过滤程序改编成一种常规诊断测试，该测试每天至少能处理10份血清，且仅需50微升血清。对108份弓形虫补体结合试验效价呈阳性的血清进行检测的结果显示，当用IIF检测全血清时，17份IgM呈阳性，而使用IgM组分检测时则有55份呈阳性。抗脱氧核糖核酸效价的血清在分离后不会产生假阳性结果，并且去除IgG可消除由类风湿因子导致的假阳性结果。一项前瞻性研究表明，弓形虫IgM可能会持续长达9个月。