黄羽扇豆（Lupinus luteus）核5S rRNA基因的组织与核苷酸序列
Organization and nucleotide sequence of nuclear 5S rRNA genes in yellow lupin (Lupinus luteus).
作者信息
Rafalski J A, Wiewiorowski M, Söll D
出版信息
Nucleic Acids Res. 1982 Dec 11;10(23):7635-42. doi: 10.1093/nar/10.23.7635.
Abstract
Genomic blots of yellow lupin (Lupinus luteus) DNA digested with restriction nucleases and probed with 32P-labelled Lupinus 5S RNA reveal that 5S DNA is organized as tandemly repeated sequences of one size class, 342 bp. The DNA is extensively methylated. Two cloned BamHI ribosomal repeats were sequenced, revealing sequence divergence within both the coding and spacer regions.
摘要
用限制性核酸酶消化的黄羽扇豆（Lupinus luteus）DNA的基因组印迹，并用32P标记的羽扇豆5S RNA进行探测，结果表明5S DNA以342 bp这一单一种类的串联重复序列形式存在。该DNA被广泛甲基化。对两个克隆的BamHI核糖体重复序列进行了测序，结果显示编码区和间隔区均存在序列差异。
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