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[乳胶试验的临床应用。I. 溶血法与乳胶法在抗链球菌溶血素抗体效价评估中的比较]

[The clinical use of latex tests. I. Comparison of hemolytic and latex methods in the evaluation of the antistreptolysin antibody titer].

作者信息

Bonamore R, Garlaschi M C, Varotto F

出版信息

Quad Sclavo Diagn. 1982 Mar;18(1):76-83.

PMID:7156334
Abstract

The TAS of 211 normo lipemic patients and of 84 hyperlipemic patients has been determined by the tube dilution hemolysis method of Rantz and Randall, the Aso Quantum hemolytic method (I.S.V.T. "Sclavo" S.p.A., Siena) and methods with streptolysin O antigen coated on the surface of biologically inert latex particles. A good correlation has been shown between all the analytical techniques considered and, moreover, the incidence of wrong positives and negatives has been showed in comparison with the hemolytic method by Rantz and Randall, considered test of reference. Finally, the greater influence of cholesterol on the classic hemolytic system, compared with the latex technique in causing an unspecific increase of TAS is proposed; this is evaluated before and after treatment of the hyperlipemic samples with ether.

摘要

采用兰茨和兰德尔的试管稀释溶血法、阿索量子溶血法(意大利锡耶纳的“Sclavo”股份公司)以及将链球菌溶血素O抗原包被在生物惰性乳胶颗粒表面的方法,测定了211名血脂正常患者和84名高脂血症患者的总抗氧化状态(TAS)。研究表明,所考虑的所有分析技术之间都具有良好的相关性,此外,与作为参考测试的兰茨和兰德尔溶血法相比,还显示了假阳性和假阴性的发生率。最后,提出与乳胶技术相比,胆固醇对经典溶血系统有更大影响,会导致TAS非特异性升高;这是在高脂血症样本用乙醚处理前后进行评估的。

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