King C R, Shinohara T, Piatigorsky J
Science. 1982 Feb 19;215(4535):985-7. doi: 10.1126/science.7156978.
The 14S messenger RNA (1300 to 1500 nucleotides) for the alpha A chain of alpha-crystallin of the mammalian lens is nearly three times larger than required to code for the polypeptide that contains 173 amino acids. As a means of accounting for this anomaly, a complementary DNA clone for the mouse alpha A-crystallin messenger RNA was constructed in pBR322 and sequenced. Derivation of the protein sequence from the nucleic acid sequence showed that mouse alpha A-crystallin is similar to that of other organisms. The messenger RNA contains 536 nucleotides located on the 3' side of the coding region, excluding the polyadenylate stretch. This 3' sequence does not encode any other crystallin and has multiple termination codons in the three possible reading frames.
哺乳动物晶状体α-晶体蛋白αA链的14S信使核糖核酸（1300至1500个核苷酸）比编码含173个氨基酸的多肽所需的长度几乎大三倍。为了解释这一异常现象，构建了一个用于小鼠αA-晶体蛋白信使核糖核酸的互补脱氧核糖核酸克隆，并将其插入pBR322载体中进行测序。从核酸序列推导蛋白质序列表明，小鼠αA-晶体蛋白与其他生物体的相似。信使核糖核酸在编码区3'侧含有536个核苷酸，不包括聚腺苷酸延伸部分。这个3'序列不编码任何其他晶体蛋白，并且在三个可能的阅读框中有多个终止密码子。
