Fougnot C, Jozefowicz M, Bara L, Samama M
Thromb Res. 1982 Oct 1;28(1):37-46. doi: 10.1016/0049-3848(82)90031-7.
In previous papers, we have described the preparation and heparin-like properties of insoluble modified polystyrene resins. We now report results about the interactions of several coagulation factors with some of these materials. Most of the non-activated factors are neither adsorbed nor modified, except factor V and prekallikrein. In contrast thrombin, antithrombin III and factor Xa adsorb on the surface of such insoluble polymers. Thrombin can be desorbed by addition of a polycationic compound. The inactivation of thrombin or factor Xa by antithrombin is catalysed by the presence in the mixture of these insoluble materials as it is with soluble heparin. The initial velocity of these reactions is second order for both types of catalysis-homogeneous or heterogeneous - as previously reported for soluble heparin. In the case of these insoluble materials, the inhibition of protease by antiprotease seems to be accelerated when complexes are formed between the polymer and the proteins.
在之前的论文中，我们已经描述了不溶性改性聚苯乙烯树脂的制备及其类肝素特性。现在我们报告几种凝血因子与其中一些材料相互作用的结果。除了因子V和前激肽释放酶外，大多数未激活的因子既不被吸附也不被修饰。相比之下，凝血酶、抗凝血酶III和因子Xa吸附在这种不溶性聚合物的表面。加入聚阳离子化合物可使凝血酶解吸。与可溶性肝素一样，这些不溶性材料混合物的存在催化抗凝血酶对凝血酶或因子Xa的灭活。这些反应的初始速度对于均相或非均相这两种催化类型来说都是二级反应，正如之前报道的可溶性肝素的情况一样。对于这些不溶性材料，当聚合物与蛋白质之间形成复合物时，抗蛋白酶对蛋白酶的抑制作用似乎会加速。
