Boisson C, Gulino D, Jozefonvicz J
Thromb Res. 1987 Apr 1;46(1):101-8. doi: 10.1016/0049-3848(87)90210-6.
In previous papers we described insoluble polystyrenes grafted with L-arginyl methyl ester (PAOM) which exhibit a high affinity for thrombin. Based on their specific interaction with the protease, the resin has been used as stationary phases for affinity chromatographic procedure of thrombin in affinity chromatography (AC) and high performance liquid affinity chromatography (HPLAC). A slight adsorption of antithrombin III (AT III) was shown in AC. In this paper we study the interaction of AT III for PAOM resin in batch procedure. The adsorption is measured using purified protein and corresponds to a monolayer adsorbed on the surface of the polymer. The affinity constant is evaluated kAT = 3.10(5) 1.M-1. Furthermore, insoluble sulfonated polystyrenes grafted with different amino acid were shown to possess an heparin-like behaviour and catalyse the generation of thrombin-AT III complex. So, compared to one of the resin previously mentioned, it could be concluded that PAOM is not heparin-like. When thrombin is adsorbed on the surface of the polymer, AT III cannot interact with the specific seryl residue of the enzyme.
在之前的论文中，我们描述了接枝有L-精氨酰甲酯（PAOM）的不溶性聚苯乙烯，其对凝血酶表现出高亲和力。基于它们与蛋白酶的特异性相互作用，该树脂已被用作亲和色谱（AC）和高效液相亲和色谱（HPLAC）中凝血酶亲和色谱法的固定相。在AC中显示出抗凝血酶III（AT III）有轻微吸附。在本文中，我们采用分批法研究了AT III与PAOM树脂的相互作用。使用纯化蛋白测量吸附情况，其对应于聚合物表面的单层吸附。评估得到亲和常数kAT = 3.10(5) 1.M-1。此外，接枝有不同氨基酸的不溶性磺化聚苯乙烯表现出类肝素行为，并催化凝血酶 - AT III复合物的生成。所以，与前面提到的一种树脂相比，可以得出PAOM不是类肝素的结论。当凝血酶吸附在聚合物表面时，AT III无法与该酶的特定丝氨酰残基相互作用。
