Borisov A B
Tsitologiia. 1982 Dec;24(12):1440-3.
The activity of 4 lysosomal enzymes (acid phosphatase, N-acetyl-beta-D-glucosaminidase, beta-D-galactosidase and acid lipase) was studied at different periods (4, 7 and 14 days) after induction of adipocyte-like differentiation in mouse L-cell culture. The differentiation was induced by adding the 60% bovine serum to the culture medium. On the 5th day after induction, an increase in enzyme activities as compared to uninduced cells, was observed. After 7 days of growth in high serum concentration medium, the increased level of activity was maintained. Cytochemical studies revealed hypertrophy of lysosomal apparatus in perinuclear region in differentiating cells. On the 15th day when the cells were filled with large lipid drops, the activity of the enzymes under study decreased in comparison with the 4 and 7-day periods. The data obtained suggest that increased endocytosis and digestion of serum lipoproteins in lysosomes with subsequent utilization of liberated components is, apparently, the source for lipid accumulation. Induction of lysosomal enzymes in connection with endocytosis and autophagocytosis in the course of adipocyte-like conversion of mouse L-cells, is discussed.
在小鼠L细胞培养中诱导脂肪细胞样分化后的不同时期（4天、7天和14天），研究了4种溶酶体酶（酸性磷酸酶、N-乙酰-β-D-氨基葡萄糖苷酶、β-D-半乳糖苷酶和酸性脂肪酶）的活性。通过向培养基中添加60%的牛血清来诱导分化。诱导后第5天，观察到与未诱导细胞相比酶活性增加。在高血清浓度培养基中生长7天后，活性水平维持升高。细胞化学研究显示，分化细胞的核周区域溶酶体装置肥大。在第15天，当细胞充满大脂滴时，与第4天和第7天相比，所研究酶的活性降低。所获得的数据表明，溶酶体中血清脂蛋白的内吞作用和消化增加，随后释放成分被利用，显然是脂质积累的来源。讨论了在小鼠L细胞脂肪细胞样转化过程中，溶酶体酶的诱导与内吞作用和自噬作用的关系。
