Kawaguchi Y
Acta Pathol Jpn. 1982 Nov;32(6):981-1002. doi: 10.1111/j.1440-1827.1982.tb02079.x.
The ultrastructural changes of the platelets stimulated by release inducers II such as thrombin, collagen, and latex particles were studied to compare the differences in morphology of release I and II. The characteristics of platelets in response to thrombin were the decrease in number of alpha-granules and marked dilation of the open canalicular system containing fibrin preceded by the disappearance of very dense granules. In the platelets in response to collagen or latex, the release of alpha-granules was initiated after taking the particles into the open canalicular system. In all of the cases, the amount of microfibrils was seen in the cytoplasm, while contraction wave of the microtubules was rare in the cytoplasm during the release and uptake of particles. Accordingly, the release of very dense granules seems to be followed by that of alpha-granules. It is suggested that the microfibrils play a more important role than the microtubules in the release II as well as release I. Furthermore, the contents of alpha-granules may be discharged via the open canalicular system on the release II by thrombin, collagen or latex particles.
研究了凝血酶、胶原蛋白和乳胶颗粒等释放诱导剂II刺激血小板后的超微结构变化，以比较释放I和释放II在形态学上的差异。血小板对凝血酶反应的特征是α颗粒数量减少，含有纤维蛋白的开放小管系统明显扩张，在此之前非常致密的颗粒消失。在对胶原蛋白或乳胶有反应的血小板中，α颗粒在颗粒进入开放小管系统后开始释放。在所有情况下，细胞质中都可见微原纤维，而在颗粒释放和摄取过程中，细胞质中微管的收缩波很少见。因此，非常致密颗粒的释放似乎先于α颗粒的释放。提示微原纤维在释放II以及释放I中比微管发挥更重要的作用。此外，在释放II时，α颗粒的内容物可能通过凝血酶、胶原蛋白或乳胶颗粒经开放小管系统排出。
