Anniko M
Laryngol Rhinol Otol (Stuttg). 1981 Jun;60(6):304-7.
The organ culture technique based on material obtained from individual human pituitary tumours represents a model for the preservation of tissue in organotypic differentiation in which the tissue can be studied in the absence of vascular, hypothalamic and other neurogenic influences. In the organ culture system, four criteria are fulfilled: (1) preserved morphology at the light and electron microscopic levels, (2) preserved synthesis/secretion of those hormones as found in vivo (3) the same reactivity in vitro to physiological stimulators/inhibitors (TRH, LH-RH and dopamine) as in vivo (4) preservation of the same cell phase in the cell cycle and the same DNA content in cells in vitro as in vivo.
基于从个体人类垂体肿瘤获取的材料的器官培养技术，代表了一种在器官型分化中保存组织的模型，在该模型中，可以在没有血管、下丘脑和其他神经源性影响的情况下研究组织。在器官培养系统中，满足四个标准：（1）在光镜和电镜水平上保持形态；（2）保持体内发现的那些激素的合成/分泌；（3）体外对生理刺激物/抑制剂（促甲状腺激素释放激素、促黄体生成素释放激素和多巴胺）的反应与体内相同；（4）体外细胞周期中的细胞期和细胞中的DNA含量与体内相同。
