不同来源人血清白蛋白样品某些物理化学性质的比较研究——I. 等离子人血清白蛋白溶液的某些物理化学性质

A comparative study of some physico-chemical properties of human serum albumin samples from different sources--I. Some physico-chemical properties of isoionic human serum albumin solutions.

作者信息

Dröge J H, Janssen L H, Wilting J

出版信息

Biochem Pharmacol. 1982 Dec 1;31(23):3775-9. doi: 10.1016/0006-2952(82)90292-1.

Abstract

Human serum albumin samples from different sources were investigated. The fatty acid content of the albumin before and after deionization on a mixed bed ion-exchange column varied from sample to sample. When an albumin sample from one source was deionized under standard conditions the amount of fatty acid bound by the albumin was reduced to a reproducible amount. In samples from different sources, however, the amount bound varied considerably. Also the isoionic pH of the albumin varied from sample to sample. This variation could be attributed to the difference in the fatty acid content and the different number of titratable histidines and acid amino-acid residues in the albumin from different sources. It can be concluded from the specific conductance of these isoionic solutions that ions other than H-, OH and protein are effectively removed by a mixed bed ion-exchange column. The specific conductance of the albumin samples is directly related to the isoionic pH. Therefore, the isoionic pH and the specific conductance of the albumins reflect the heterogeneity of the albumin samples with respect to their primary structure and fatty acid content.

摘要

对来自不同来源的人血清白蛋白样本进行了研究。在混合床离子交换柱上进行去离子化前后，白蛋白的脂肪酸含量因样本而异。当一个来源的白蛋白样本在标准条件下去离子化时，白蛋白结合的脂肪酸量会减少到可重现的量。然而，在来自不同来源的样本中，结合的量差异很大。此外，白蛋白的等离子pH值也因样本而异。这种差异可归因于不同来源白蛋白中脂肪酸含量的差异以及可滴定组氨酸和酸性氨基酸残基数量的不同。从这些等离子溶液的电导率可以得出结论，混合床离子交换柱能有效去除除H⁺、OH⁻和蛋白质以外的离子。白蛋白样本的电导率与等离子pH值直接相关。因此，白蛋白的等离子pH值和电导率反映了白蛋白样本在一级结构和脂肪酸含量方面的异质性。