糖化血红蛋白的定量。在pH 5的样品溶血过程中去除不稳定糖化血红蛋白。
Quantitation of glycosylated hemoglobin. Elimination of labile glycohemoglobin during sample hemolysis at pH 5.
作者信息
Bissé E, Berger W, Flückiger R
出版信息
Diabetes. 1982 Jul;31(7):630-3. doi: 10.2337/diab.31.7.630.
PMID:7160540
Abstract
A simple method for the elimination of labile glycohemoglobin in the chromatographic quantitation of glycosylated hemoglobin is described. Use is made of the instability of Schiff base adducts in acidic solution. Erythrocytes are lysed with a pH 5 buffer. At this pH dissociation reaches completion during sample preparation.
摘要
描述了一种在糖化血红蛋白色谱定量分析中消除不稳定糖化血红蛋白的简单方法。利用席夫碱加合物在酸性溶液中的不稳定性。用pH 5的缓冲液裂解红细胞。在此pH值下，解离在样品制备过程中完成。
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