Litman G W, Stolen J S, Sarvas H O, Mäkelä O
J Immunogenet. 1982 Dec;9(6):465-74. doi: 10.1111/j.1744-313x.1982.tb01008.x.
Heterodontus francisci (horned shark) and Pseudopleuronectes americanus (winter flounder) were immunized with furyl-oxazolone (furyl-Ox) and phenyl-oxazolone (phenyl-Ox) coupled either to bacteria or protein carriers. The antibodies produced were measured by inactivation of furyl- or phenyl-Ox conjugated bacteriophage, and their affinity and fine specificity were estimated by inhibition of phage inactivation with a series of structurally related hapten analogues. In both species, post-immunization peak titres were 100 to 2000 times higher than preimmunization titres. A number of unique features distinguished Heterodontus antibodies from Pseudopleuronectes or mammalian antibodies. Heterondontus antibodies exhibited a lower affinity for the immunizing hapten (furyl-Ox or phenyl-Ox) and a reduced ability to distinguish the homologous immunogenic hapten from its structural analogues. In addition, Heterodontus antibodies exhibited a lower level of inter-individual variation in affinity and fine specificity than did Pseudopleuronectes or mammalian IgM antibodies; this was especially prominent in anti-furyl-Ox responses. Typically the affinity and fine specificity of Heterodontus antibodies did not change over the 146-day period of immunization and were not influenced by the nature of the carrier. The implications of these findings in terms of the phylogenetic origins of antibody diversity are discussed.
用与细菌或蛋白质载体偶联的呋喃基恶唑酮（furyl - Ox）和苯基恶唑酮（phenyl - Ox）对角鲨（Heterodontus francisci）和美洲拟庸鲽（Pseudopleuronectes americanus）进行免疫。通过呋喃基或苯基 - Ox共轭噬菌体的失活来测量产生的抗体，并通过一系列结构相关的半抗原类似物抑制噬菌体失活来估计它们的亲和力和精细特异性。在这两个物种中，免疫后的峰值滴度比免疫前的滴度高100至2000倍。角鲨的抗体与美洲拟庸鲽或哺乳动物的抗体有许多独特的特征。角鲨抗体对免疫半抗原（呋喃基 - Ox或苯基 - Ox）的亲和力较低，区分同源免疫原性半抗原与其结构类似物的能力也较低。此外，与美洲拟庸鲽或哺乳动物的IgM抗体相比，角鲨抗体在亲和力和精细特异性方面的个体间差异水平较低；这在抗呋喃基 - Ox反应中尤为突出。通常，角鲨抗体的亲和力和精细特异性在146天的免疫期间没有变化，也不受载体性质的影响。讨论了这些发现对于抗体多样性系统发育起源的意义。
