用于鉴定生物材料中巴比妥酸盐的快速烷基化法。

Flash alkylation for the identification of barbiturates in biological material.

作者信息

Gambaro V, Mariani R, Marozzi E

出版信息

J Anal Toxicol. 1982 Nov-Dec;6(6):321-3. doi: 10.1093/jat/6.6.321.

DOI:10.1093/jat/6.6.321

Abstract

The gas chromatographic identification of barbiturates isolated from biological material is not optimal, and due to their polar nature, it is difficult to obtain good resolution on most columns. To improve resolution, flash alkylation appeared to be a reasonable alternative. Methyl, ethyl, and butyl derivatives were prepared for various drugs found in the "acid-neutral" extract and the Kovats retention indices (Ir) were determined on SE-30, OV-17, and SP-2250. The Ir of the alkylated derivatives of the barbiturates are highly reproducible. One column and two alkylated derivatives can be used for the qualitative analysis of barbiturates in extracts of biological material, even when the Stas-Otto extract is used.

摘要

从生物材料中分离出的巴比妥类药物的气相色谱鉴定并不理想，由于其极性，在大多数色谱柱上难以获得良好的分离度。为提高分离度，快速烷基化似乎是一种合理的选择。针对“酸中性”提取物中发现的各种药物制备了甲基、乙基和丁基衍生物，并在SE - 30、OV - 17和SP - 2250上测定了科瓦茨保留指数（Ir）。巴比妥类药物烷基化衍生物的Ir具有高度重现性。即使使用斯塔斯 - 奥托提取物，一根色谱柱和两种烷基化衍生物也可用于生物材料提取物中巴比妥类药物的定性分析。