体外人血浆中的荧光素。

Fluorescein in human plasma in vitro.

作者信息

Lund-Andersen H, Krogsaa B

出版信息

Acta Ophthalmol (Copenh). 1982 Oct;60(5):701-8. doi: 10.1111/j.1755-3768.1982.tb06730.x.

DOI:10.1111/j.1755-3768.1982.tb06730.x

Abstract

A method to determine fluorescein in human plasma is described. By aid of ultrafiltration a separation between fluorescein bound to plasma proteins and fluorescein free in the water is obtained. Both fractions are quantitated. Fluorescein is bound to plasma proteins. The protein binding is reversible, not sensitive to practically appearing changes in pH, temperature and gas tensions. For a normal person it appeared that at a total plasma concentration between (10(-6) - 10(-4))g . ml-1 approximately 15% was free while at a total concentration of 10(-3) g . ml-1 45% was free, pointing towards a limited amount of protein binding sites.

摘要

本文描述了一种测定人血浆中荧光素的方法。借助超滤技术，可将与血浆蛋白结合的荧光素和水中游离的荧光素分离。对这两个部分进行定量分析。荧光素与血浆蛋白结合。这种蛋白结合是可逆的，对实际出现的pH值、温度和气体张力变化不敏感。对于一个正常人而言，当血浆总浓度在(10⁻⁶ - 10⁻⁴)g·ml⁻¹之间时，约15%为游离状态；而当总浓度为10⁻³g·ml⁻¹时，45%为游离状态，这表明蛋白结合位点数量有限。