Ianacone D C, Felberg N T, Federman J L
Arch Ophthalmol. 1980 Sep;98(9):1643-5. doi: 10.1001/archopht.1980.01020040495022.
Aliquots of normal human plasma that had been incubated with tritiated fluorescein were examined for radioactive binding to proteins. Samples were fractionated by polyacrylamide gel electrophoresis (PAGE) and gel filtration. Aliquots removed between zero time and two hours showed no specific radioactive binding peaks by either method. Tritiated fluorescein ran well ahead of all proteins at all times on PAGE and well after the protein eluted from a fractionating column (Sephadex G-75). Ten minutes after intravenous injections, PAGE of plasma from three patients undergoing fluorescein angiography with nonradioactive dye showed the only fluorescent band migrating ahead of all protein bands. These methods failed to demonstrate specific binding of tritiated fluorescein to normal human plasma proteins. We conclude that during angiography, fluorescein exists in plasma as an unbound molecule or is so weakly associated with plasma proteins as to be undetectable by the methods used.
对与氚化荧光素孵育过的正常人血浆等分试样进行放射性结合蛋白检测。样品通过聚丙烯酰胺凝胶电泳（PAGE）和凝胶过滤进行分离。在零时间至两小时之间取出的等分试样，通过任何一种方法均未显示出特异性放射性结合峰。在PAGE上，氚化荧光素在所有时间都比所有蛋白质迁移得快，并且在从分馏柱（葡聚糖凝胶G - 75）洗脱蛋白质之后很久才迁移。静脉注射十分钟后，对三名接受非放射性染料荧光素血管造影的患者的血浆进行PAGE分析，结果显示唯一的荧光带在所有蛋白带之前迁移。这些方法未能证明氚化荧光素与正常人血浆蛋白的特异性结合。我们得出结论，在血管造影过程中，荧光素在血浆中以未结合分子的形式存在，或者与血浆蛋白的结合非常弱，以至于所用方法无法检测到。
