Schultz E, Lipton B H
Mech Ageing Dev. 1982 Dec;20(4):377-83. doi: 10.1016/0047-6374(82)90105-1.
Skeletal muscle satellite cells were isolated from soleus and extensor digitorum longus muscles of Sprague-Dawley rats at selected ages between 6 days and 30 months and grown in cell culture. Cells at each donor age were monitored individually to determine the number of progeny they were capable of producing. Under identical in vitro conditions, the average number of progeny produced was inversely proportional to donor age. Hence, the proliferation potential of satellite cells decreases with age.
从6日龄至30月龄的特定年龄段的Sprague-Dawley大鼠的比目鱼肌和趾长伸肌中分离出骨骼肌卫星细胞，并在细胞培养中生长。对每个供体年龄的细胞进行单独监测，以确定它们能够产生的后代数量。在相同的体外条件下，产生的后代平均数量与供体年龄成反比。因此，卫星细胞的增殖潜力随年龄增长而降低。
