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[成红细胞岛的分离培养及其中央巨噬细胞的分离技术]

[Technique for isolation and culture of erythroblastic islands and separation of their central macrophage].

作者信息

Zakharov Y M, Prenant M

出版信息

Nouv Rev Fr Hematol (1978). 1982;24(6):363-7.

PMID:7167386
Abstract

Erythroblastic islands (EI) were isolated using the adherence of their central macrophage to glass or plastic. The EI obtained were cultured and erythroblasts achieved a complete maturation within 72 h of incubation. When cultured in serum-free medium, the central macrophages were rapidly isolated, by detachment from their erythroblasts. Isolation and culture of central macrophages allow the study of their physiological characteristics and particularly the effect of their supernatant on erythropoïesis.

摘要

通过其中心巨噬细胞对玻璃或塑料的黏附作用分离出成红细胞岛(EI)。分离得到的EI进行培养,成红细胞在培养72小时内实现完全成熟。当在无血清培养基中培养时,中心巨噬细胞会迅速从其成红细胞上脱离而被分离出来。中心巨噬细胞的分离和培养有助于研究其生理特性,特别是其培养上清液对红细胞生成的影响。

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