Strobel S, Ferguson A, Anderson D M
Toxicol Lett. 1982 Dec;14(3-4):247-52. doi: 10.1016/0378-4274(82)90059-5.
An inexpensive animal model is described, for investigation of the immunogenicity of substances such as food additives. Inbred mice were immunised with antigen emulsified in complete Freund's adjuvant, and specific cell-mediated immunity subsequently measured by a footpad swelling test. This method has been applied in an investigation of the immunogenicity of the exudate gums, gum arabic, gum karaya and gum tragacanth. These substances are capable of eliciting an immune response which is comparable to the specific immune responses elicited by a protein antigen, e.g. hens' egg ovalbumin. Purification of commercially available gum preparations led to a significant (P less than 0.005) reduction of the immune response under in vivo test conditions.
描述了一种用于研究食品添加剂等物质免疫原性的廉价动物模型。将近交系小鼠用完全弗氏佐剂乳化的抗原进行免疫，随后通过足垫肿胀试验测量特异性细胞介导免疫。该方法已应用于对渗出树胶、阿拉伯胶、刺梧桐树胶和黄芪胶免疫原性的研究。这些物质能够引发与蛋白质抗原（如鸡卵白蛋白）引发的特异性免疫反应相当的免疫反应。在体内试验条件下，市售树胶制剂的纯化导致免疫反应显著降低（P小于0.005）。
