Salo M, Lassila O, Viljanen M, Eskola J, Kangas L
Acta Pathol Microbiol Scand C. 1978 Jun;86C(3):105-8. doi: 10.1111/j.1699-0463.1978.tb02566.x.
The effect of a single halothane anaesthesia on the secondary antibody response and the lymphocyte response in vitro to phytohaemagglutinin (PHA) and concanavalin A(Con A) was studied. The antigens used were bovine serum albumin (BSA) and killed Brucella abortus organisms (Brucella). IgG and IgM antibodies against these antigens were quantified by solid-phase radioimmunoassay. Lymphocyte transformation was studied by a whole blood micromethod. The chickens were anaesthetised for two hours with 2% v/v halothane. The halothane concentration of the blood was measured by gas chromatography at the end of the anaesthesia. Halothane anaesthesia had no effect on the secondary antibody response against BSA and Brucella. In contrast, it caused a significant decrease in the lymphocyte response to PHA and Con A. This suppression was transient, however, and full recovery of the lymphocyte function was observed by the first day after anaesthesia.
研究了单次氟烷麻醉对二次抗体反应以及体外淋巴细胞对植物血凝素（PHA）和刀豆球蛋白A（Con A）反应的影响。所用抗原为牛血清白蛋白（BSA）和灭活的流产布鲁氏菌（布鲁氏菌）。通过固相放射免疫测定法对针对这些抗原的IgG和IgM抗体进行定量。采用全血微量法研究淋巴细胞转化。用2%（v/v）氟烷对鸡麻醉两小时。麻醉结束时通过气相色谱法测量血液中的氟烷浓度。氟烷麻醉对针对BSA和布鲁氏菌的二次抗体反应没有影响。相比之下，它导致淋巴细胞对PHA和Con A的反应显著降低。然而，这种抑制是短暂的，麻醉后第一天观察到淋巴细胞功能完全恢复。
