[Effect of normoxic and anoxic liver perfusion as well as ischemic liver storage on parameters of mitochondrial respiration and swelling].

Using the model of Hb-free perfused rat liver, the influence of normoxic or anoxic perfusion, as well as ischemic storage of the liver on parameters of respiration and on swelling has been studied comparatively. The system used for Hb-free perfusion ensures at normoxic perfusion of the liver the functional integrity of isolated mitochondria for a period of investigation of 180 min. Normothermal ischemia of the liver leads, already after 20 min, to a significant decline of the ADP/O quotient and of the respiration control coefficient of the isolated mitochondria, as well as to mitochondrial swelling. Hypothermal ischemia of the liver (25 degrees C) clearly diminishes the mitochondrial functional loss. As far as the liver disposes of an endogenous glycogen store, the integrity of the mitochondria isolated after anoxic liver perfusion (25 degrees C) is retained and longer than after hypothermal ischemia of the liver. Mitochondria isolated after anoxic perfusion from glycogen-deprived liver swell and uncouple as fast as mitochondria prepared after ischemic storage of the glycogen-containing liver. Normoxic reperfusion of the ischemically pre-damaged liver leads to partial recovery of the mitochondria, if reoxygenation of the liver is started no later than after 80 min of ischemic storagel.