Marcsek Z, Menyhárt J
Acta Physiol Acad Sci Hung. 1982;59(4):349-58.
This paper reports on partial purification and characterization of a natural (endogenous) factor capable of inhibiting in vivo DNA synthesis in mouse pneumocytes in a tissue-specific manner. By using a combination of ultrafiltration and various chromatographic techniques, the active agent has been partially purified from aqueous extracts of both bovine and rat pulmonary tissue. The factor responsible for the observed effect was found to be a heat labile compound, most likely a protein of approx. 40 000 molecular weight. Its chemical and physicochemical properties determined so far, and also the manner of its biological action implies that this lung tissue derived agent might be an endogenous proliferation inhibitor with a chalone-like character operating in the pulmonary epithelial cells.
本文报道了一种能够以组织特异性方式抑制小鼠肺细胞体内DNA合成的天然（内源性）因子的部分纯化和特性研究。通过超滤和各种色谱技术的结合，已从牛和大鼠肺组织的水提取物中部分纯化了活性剂。发现导致观察到的效应的因子是一种热不稳定化合物，很可能是一种分子量约为40000的蛋白质。到目前为止所确定的其化学和物理化学性质，以及其生物学作用方式表明，这种源自肺组织的因子可能是一种在肺上皮细胞中起作用的具有类抑素特性的内源性增殖抑制剂。
